Gabes, Tunisia
21:09 UTC
About
I’m a medical biotechnology graduate from the Higher Institute of Medical Technologies of Tunis. My work centers on molecular biology, virology, and bioinformatics, with research experience at the Pasteur Institute of Tunis focusing on rabies virus detection using real-time RT-PCR. I’m passionate about advancing diagnostic technologies and using biotechnology to make healthcare more precise, accessible, and innovative.
Experience
Intern - Rabies Laboratory
Feb 2025 - May 2025
Pasteur Institute of Tunis
Processed 81 brain tissue samples under controlled temperature conditions (+4°C, 37°C, room temperature) for 1, 3, and 7 days
Performed direct immunofluorescence assay (IFD) using fluorescent antibodies for rabies virus detection
Extracted viral RNA using QIAGEN and PROMEGA kits and conducted Real-Time RT-PCR using the LightCycler 480 systems
Achieved 100% sensitivity and specificity for rabies virus detection in putrefied samples
Performed Real-Time RT-PCR, starting with preparation of the reaction mix, arranging samples on PCR plates, and finishing with analysis using the LightCycler 480 system
Conducted bioinformatics analysis using BioEdit and MEGA: identified mutations, constructed phylogenetic trees, and assessed rabies virus strain diversity in Tunisia
Intern - Virology Laboratory
Jun 2024 - Jul 2024
Pasteur Institute of Tunis
Retrieved and organized approximately 30 SARS-CoV-2 samples from the freezer, ensuring accurate tracking across multiple tubes using a detailed log sheet
Defrosted all 30 samples to preserve viral RNA integrity
Performed conventional and real-time PCR to detect and quantify viral RNA
Purified RNA for downstream molecular analyses, such as standard sequencing
Intern - Biochemistry Laboratory
Jun 2023 - Jul 2023
University Hospital of Gabes
Collected 130 patient blood samples using sterile techniques, ensuring accurate labeling and identification
Separated plasma from cellular components via centrifugation and transferred to secondary tubes for biochemical analysis
Performed quantitative measurements of blood components (glucose, urea, creatinine, liver enzymes) using an automated analyzer
Verified instrument calibration and reagent availability to ensure accurate and reliable test results
Education
Bachelor in Medical Biotechnology
2022 - 2025
Higher Institute of Medical Technologies - Manar Campus
Integrated Preparatory Cycle in Health Biotechnology Engineering
Grade: 13.82
Courses: Molecular Biology, Microbial Genetics, Human Genetics, Microbiology, Biochemistry, Computer Science, Mathematics, Fluid Mechanics
Thesis topic: Diagnosis of rabies from putrefied samples using molecular biology: application of real-time RT-PCR
Certifications
Software & Tools
Python
R
Next.js
MEGA
BioEdit
SwissDock
SwissDock
PyMol
AutoDock Vina
Languages
Arabic
Native
English
C1
French
B2
German
B1